FIG. 1.

Physical map of the pchDCBA-pchR-pchEFGHI-fptA region of P. aeruginosa PAO1. The pchDCBA operon and the pchR, pchEF, and fptA genes have been described previously (1, 15, 27, 32). The ΩSm/Sp cassette present in mutants PAO6333, PAO6335, PAO6336, PAO6337, and PAO6338 is designated by Ω. Deletions are indicated by Δ. Subclones used for complementation experiments were constructed such that the respective vector promoter (indicated by a triangle) was located upstream of pchE, pchG, pchD, and entD. The restriction enzymes used for subcloning experiments are indicated. Note that for SalI, PvuII, MunI, and RsaI, only the locations relevant to this study are shown.