Figure 2.

A periodic hydrostatic pressurization system. Our experimental system was composed of a pressure chamber containing a cell culture dish, a compressor for collecting 37°C air containing 5% CO₂ from the incubator and transferring it to an air tank, a flow sensor and regulator for controlling the flow of pressurized air into the pressure chamber, and a computer for setting the desired magnitude and frequency of the periodic pressurization. Pressurization to 110-180 kPa at a frequency of 0.002 Hz is illustrated.