TABLE 1.
S. cerevisiae strains used in this study
| Straina | Genotypeb | Source or reference |
|---|---|---|
| 463-1C | MATaleu2 his3 trp1 ura3 | K. Tatchelc |
| 463-1D | MATα leu2 his3 trp1 ura3 | K. Tatchel |
| 463-1C/463-1D | MATa/MATα leu2/leu2 his3/his3 trp1/trp1 ura3/ura3 | K. Tatchel |
| CJ2-A | MATaupc2-1 leu2 his3 trp1 ura3 | 4 |
| CJ625 | MATaupc2::URA3 leu2 his3 trp1 ura3 | 4 |
| H1B | MATα ylr228c::LEU2 leu2 his3 trp1 ura3 | This study |
| H1B (+) | MATα ylr228c::LEU2 leu2 his3 trp1 ura3 | This study |
| KS93 | MATaupc2-1 ylr228c::LEU2 leu2 his3 trp1 ura3 | This study |
| KS93 (+) | MATaupc2-1 ylr228c::LEU2 leu2 his3 trp1 ura3 | This study |
| KS94 (+) | MATaupc2::URA3 ylr228c::LEU2 leu2 his3 trp1 ura3 | This study |
| H1B (++) | MATα ylr228c::LEU2 leu2 his3 trp1 ura3 | This study |
| KS122 (++) | MATaupc2-1 ylr228c::LEU2 leu2 his3 trp1 ura3 | This study |
| KS119 (++) | MATaupc2::URA3 ylr228c::LEU2 leu2 his3 trp1 ura3 | This study |
a
+, Strain is transformed with the plasmid YEp351 containing the YLR228c point mutation; ++, strain is transformed with the plasmid pRS313 containing the YLR228c point mutation.
b
upc2 = YDR213w.
c
Kelly Tatchel, Louisiana State University Medical Center, Shreveport, La.