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Aerobic sterol uptake (low-copy vector)
| Straina | Genotype | Sterol uptakeb |
|---|---|---|
| 463-1C | Wild type | 1 |
| CJ2-A | upc2-1 | 11.4 |
| H1B | ylr228c::LEU2 | 1.5 |
| H1B (++) | ylr228c::LEU2 | 1.2 |
| KS122 (++) | ylr228c::LEU2 upc2-1 | 9.1 |
| KS119 (++) | ylr228c::LEU2 upc2::URA3 | 1.5 |
++, Strain was transformed with the pRS313 plasmid containing the YLR228c point mutation.
Sterol uptake is represented as the fold difference compared to the wild type from an experiment performed in triplicate.
