KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| 12G10 | (Mould et al., 1995) Hybridoma from Martin Humphries | Produced from hybridoma in lab |
| 9EG7 | (Lenter et al., 1993) Hybridoma from Dietmar Vestweber | Produced from hybridoma in lab |
| SG/19 | (Miyake et al., 1992) Hybridoma from Jun Takagi | Produced from hybridoma in lab |
| MBC319.4 | (Zhang et al., 2013) Hybridoma from Richard Aster | Produced from hybridoma in lab |
| LIBS1 | (Frelinger et al., 1990) Hybridoma from Mark Ginsberg | Produced from hybridoma in lab |
| AP5 | (Kekomaki et al., 1991) Antibody from Dan Bougie, Versiti | Cat#153747 |
| HUTS4, HUTS-21 | (Luque et al., 1996) Hybridoma from Carlos Cabamas | Produced from hybridoma in lab |
| mAb16 | (Akiyama et al., 1989) Hybridoma from Kenneth Yamada | Produced from hybridoma in lab |
| 17E6 | (Mitjans et al., 1995) Antibody from Simon Goodman, Merck KGA | |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Thrombin | Sigma-Aldrich | Cat#10602400001 |
| Prostaglandin E1 | Sigma-Aldrich | Cat#P5515 |
| FITC-echistatin | US Biological | Cat#E0256–01A.1 |
| FITC-LDVP | Tocris Bioscience | Cat#4577 |
| Alexa Fluor™ 647 NHS Ester | Thermo Fisher Scientific | Cat#A20006 |
| Alexa Fluor™ 488 NHS Ester | Thermo Fisher Scientific | Cat#A20000 |
| Dodecapeptide (HHLGGAKQAGDV) | Phoenix Pharmaceuticals | Cat#025–35 |
| Tirofiban | Merck | CAS 144494-65-5 |
| Eptifibatide | Sigma-Aldrich | SML1042; CAS 188627-80-7 |
| Orbofiban (active form) | Pfizer | CAS 199530-77-3 |
| Roxifiban (active form) | This paper | CAS 170725-15-2 |
| Lotrafiban (active form) | This paper | CAS 171049-14-2 |
| Lamifiban | Roche | CAS 144412-49-7 |
| Sibrafiban (active form) | Roche | CAS 144412-18-0 |
| Fradafiban (active form) | This paper | CAS 148396-36-5 |
| EF-5154 | Meiji Seika Pharma | CAS 177276-17-4 |
| UR-2922 | This paper | CAS 220386-56-1 |
| BMS4 | This paper | CAS 270593-59-4 |
| BMS4–1 | This paper | CAS 270088-90-9 |
| BMS4–3 | This paper | N/A |
| BMS4–2 | This paper | CAS 270088-87-4 |
| GR144058 | Sigma-Aldrich | G6418; CAS 150040-23-6 |
| Gantofiban | This paper | CAS 167364-04-7 |
| Gantofiban analog | This paper | CAS 167364-14-9 |
| RUC-4 | Barry Coller | CAS 1448313-27-6 |
| M-tirofiban (both isomers) | This paper | (S)-isomer: CAS 2410607-77-9; (R)-isomer: N/A |
| Firategrast | Apexbio | Cat#A3415 CAS 402567-16-2 |
| Ro 29 | Roche | CAS 1421673-59-7 |
| DS 13d | Tocris Bioscience | Cat#TCS 2314 CAS 317353-73-4 |
| DS 13g | This paper | CAS 819078-63-2 |
| Deposited Data | ||
| Drug-bound αIIbβ3 structures | This paper | PDB accession IDs for structures are in Table S1 |
| Drug-bound αIIbβ3 structure diffraction images | SBGRID accession IDs for diffraction images are in Table S1: https://data.sbgrid.org/data/ | |
| Experimental Models: Cell Lines | ||
| Jurkat | ATCC | Cat#TIB-152 |
| Expi293F | Thermo Fisher Scientific | Cat# A14527 |
| Expi293F α5&αV knock-out cells | This paper | N/A |
| HEK293 expression αllbβ3 with GAAKR mutant | (Luo et al., 2004) | N/A |
| Recombinant DNA | ||
| PD2529 CAG αIIb full length | This paper | Addgene Plasmid #189796 |
| PD2529 CAG β3 full length | This paper | Addgene Plasmid #189797 |
| PD2529 CAG β3_N305T full length | This paper | Addgene Plasmid #189798 |
| pSpCas9n(BB)-2A-puro | (Ran et al., 2013) | Addgene Plasmid #4814 |
| Software and Algorithms | ||
| Mathematica | Wolfram Mathematica version 12 | https://www.wolfram.com/mathematica/ |
| Function F in Eq. 4 and its derivation | This paper | Mathematica file Equation4.nb |
| Phenix | (Adams et al., 2010) | http://www.phenix-online.org |
| XDS | (Kabsch, 2010) | xds.mpimf-heidelberg.mpg.de |
| COOT | (Emsley et al., 2010) | http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot |
| Maestro, Prime, and Epik | Schrödinger Inc. | https://www.schrodinger.com |
| PyMOL | Schrödinger Inc. | https://pymol.org/2/ |
| SciFinder | https://scifinder-n.cas.org | https://scifinder-n.cas.org |
| Plot2 | Apple Inc. App store | https://plotdoc.micw.org |
| Prism9 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |