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. 2022 Sep 22;18:355. doi: 10.1186/s12917-022-03445-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — RBCs serial dilutions (1:2) in canine sera (controlled haemolysis). A Visual inspection and measurement of absorbance of haemoglobin at 414 nm (A414). B Haemolysis series showing a linear association between A414 and the increase of red/pink colouration in the serial dilutions of RBCs. C RBCs serial dilutions (% v/v) associated with their respective A414 value. Absorbance values represent mean of triplicate measurements ± SD. RBCs: red blood cells