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. 2022 Sep 22;18:355. doi: 10.1186/s12917-022-03445-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Absolute quantification of miRNAs sensitive to haemolysis in whole serum (WS) and serum-derived extracellular vesicles (EV) groups, in patients’ sera with different degrees of haemolysis (validation experiments). Grey columns in the background depict increasing levels of haemolysis, measured using the haemoglobin absorbance at 414 nm. miRNA concentration was measured by means of absolute quantification, converting Ct values into fM miRNA based on miRNA-specific standard curves. Experiments were performed in triplicate and markers show the mean ± SD (bars)