Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The primer sequences for the real time qPCR analysis.
| Genes | Type | Sequences (5′–3′) | Temperature (C) |
|---|---|---|---|
| 16 sRNA (reference gene) | Forward Reverse |
AGAGTTTGATCMTGGCTCAG CTGCTGCSYCCCGTAG |
50 52 |
| acrA gene (target gene) | Forward Reverse |
TTGAAATTCAGGAT CTTAGCCCTAACAGGATGTG |
53 57.2 |
