Table 4.
CPP bioactive peptide conjugates for intracellular targets II. Activity probabilities *.
| No. | Peptide Name/Ref. | Extended CPP at the N or C-terminal * | CPP | Anti-Microbial | Anti-Cancer | Anti-Viral | Anti-Fungal | Anti-Inflamm. | Hemo-lytic | Toxicity/ Score |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | KW [168]/E | KRKRWHW | 0.99/H | 1.00/ND | 0.98/0.98 | 0.8/0.4/1.0 | 0.38/ND | 0.62/0.54 | 0.01 | −0.93 |
| 2 | Ribosomal-homing- peptide (RHP)-KW [54]/DJ |
YKWYYRGAA-KRKRWHW | 0.93/H | 0.97/1.00 | 0.99/0.98 | 1.0/0.9/0.8 | 0.62/0.74 | 0.51/0.61 | 0.02 | −0.58 |
| 3 | L-K6V1 temp [112]-KW/DJ | IKKIVSKIKKLLK-GG-KRKRWHW | 0.89/H | 0.98/1.00 | 1.0/0.98 | 1.0/1.0/0.2 | 0.95/1.00 | 0.59/0.66 | 0.02 | −1.35 |
| 4 | CAMEL [148]-KW/DJ | KWKLFKKIGAVLKVL-KRKRWHW | 0.92/H | 1.00/1.00 | 0.99/0.98 | 1.0/1.0/1.0 | 0.78/0.99 | 0.61/0.67 | 0.94 | −0.90 |
| 5 | R2-chensenin [149]-KW/DJ | VWRRWRRFWRR-GG-KRKRWHW | 0.99/H | 1.00/0.99 | 0.95/0.98 | 1.0/0.9/1.0 | 0.27/1.00 | 0.66/0.70 | 0.15 | −1.03 |
| 6 | ZY4 [150]-KW/DJ | VCKRWKKWKRKWKKWCV-GG- KRKRWHW |
0.95/H | 1.00/1.00 | 0.99/0.98 | 1.0/0.9/1.0 | 0.34/1.00 | 0.58/0.68 | 0.23 | −0.36 |
| 7 | Puroindoline [151]-KW/DJ | FPVTWRWWKWWKG-G-KRKRWHW | 0.88/H | 1.00/0.99 | 0.98/0.98 | 1.0/0.9/1.0 | 0.46/0.99 | 0.64/0.65 | 0.67 | −0.83 |
| 8 | Novispirin [152]-KW/DJ | KNLRIIRKGIHIIKKY-GG-KRKRWHW | 0.90/L | 0.98/1.00 | 0.99/0.98 | 1.0/1.0/1.0 | 0.96/1.00 | 0.63/0.63 | 0.43 | −0.90 |
| 9 | BP33 [144]-KW/DJ | LKLFKKILKVL-G-KRKRWHW | 0.93/H | 1.00/1.00 | 0.99/0.98 | 1.0/1.0/1.0 | 0.92/1.00 | 0.62/0.70 | 0.82 | −1.19 |
| 10 | T2R1 [88]-KW/DJ | RHHWRRYARIGFRAVRTVIGK- KRKRWHW |
0.94/H | 0.99/0.94 | 0.92/0.98 | 1.0/1.0/0.8 | 0.71/0.86 | 0.66/0.62 | 0.12 | −1.09 |
| 11 | DiPGLa-H [153]-KW peptide/DJ | KIAKVALKALKIAKVALKAL- KRKRWHW |
0.92/L | 0.98/1.00 | 0.99/0.98 | 1.0/1.0/1.0 | 0.97/0.99 | 0.49/0.63 | 0.94 | −0.92 |
| 12 | Neoepitope4-WFR8 [169]/DJ | VLSHGSFVM-GG-RRWFRRRRRR | 0.89/H | 0.89/0.93 | 0.59/0.98 | 1.0/0.8/0.1 | 0.62/0.76 | 0.62/0.62 | 0.43 | −1.22 |
| 13 | WFR8 -tumor homing [170]/DJ | RRWFRRRRRR-GG-IFLLWQR | 0.99/H | 1.00/0.78 | 0.48/0.98 | 1.0/0.5/0.8 | 0.46/0.96 | 0.63/0.63 | 0.07 | −1.26 |
| 14 | BP100 [155]-KW/DJ | KKLFKKILKYL-GG-KRKRWHW | 0.93/H | 1.00/1.00 | 1.0/0.98 | 1.0/1.0/1.0 | 0.94/0.99 | 0.58/0.65 | 0.61 | −1.35 |
| 15 | Mitoch. target. [157]-KW/DJ | KLLNLISKLF-GGG-KRKRWHW | 0.80/L | 0.97/1.00 | 0.74/0.98 | 1.0/1.0/0.9 | 0.91/0.98 | 0.63/0.67 | 0.41 | −1.29 |
| 16 | Nosangiotide [158]-KW/DJ | RKKTFKEVANAVKISA-GG-KRKRWHW | 0.69/L | 0.85/0.93 | 0.87/0.97 | 0.5/0.9/0.9 | 0.88/0.84 | 0.69/0.59 | 0.01 | −1.03 |
| 17 | Adepantin-1A [88]-WFR8/DJ | GIKKAVGKALKGLKGLLKALGES -GG-RRWFRRRRRR |
0.80/L | 1.00/0.99 | 0.95/0.98 | 1.0/1.0/1.0 | 0.98/1.00 | 0.60/0.66/1.30566 | 0.66 | −1.46 |
| 18 | WFR8-adepantin-1A/DJ |
RRWFRRRRRR- GIKKAVGKALKGLKGLLKALGES |
0.86/L | 1.00/1.00 | 0.95/0.96 | 1.0/1.0/1.0 | 0.97/0.99 | 0.62/0.62/1.36028 | 0.63 | −1.56 |
| 19 | KW-pexiganan-L18 [88]/DJ |
KRKRWHW- GIGKFLKKAKKFGKAFVLILKK |
0.87/H | 0.99/1.00 | 1.0/0.98 | 1.0/0.9/1.0 | 0.99/0.99 | 0.53/0.64 | 0.81 | −1.04 |
| 20 | RtLyp-1-flexampin [114]/DJ | RCGNKRTR- GIKKWVKGVAKGVAKDLAKKIL |
0.59/L | 0.92/1.00 | 1.0/0.97 | 1.0/1.0/1.0 | 1.00/1.00 | 0.44/0.63 | 0.68 | −0.74 |
| 21 | Zyk-1- [88]-WFR8/DJ | GIGREIIKKIIKKIGKKIGRII -GG-RRWFRRRRRR |
0.89/H | 1.00/1.00 | 0.99/0.98 | 1.0/1.0/1.0 | 0.96/0.99 | 0.60/0.66 | 0.88 | −1.18 |
| 22 | MG2-bombesin [171]/E | GIGKFLHSAKKFGKAFVGEIMNS-GG- QRLGNQWAVGHLM |
0.30 | 0.83/0.85 | 0.86/0.54 | 1.0/0.9/0.9 | 0.97/0.41 | 0.53/0.55 | ND | −0.97 |
| 23 | MG2-pAntp [172]/E | GIGKFLHSAKKFGKAFVGEIMNS-GG- KKWKMRRNQFWVKVQRG |
0.52/L | 0.95/1.00 | 0.93/0.81 | 1.0/1.0/1.0 | 0.99/0.81 | 0.56/0.52 | ND | −0.68 |
| 24 | DP1 [173]/E | RRQRRTSKLMKR-GG- KLAKLAKKLAKLAK |
0.95/L | 0.84/1.00 | 0.91/0.98 | 0.7/0.7/0.2 | 0.95/0.55 | 0.50/0.65 | 0.03 | −0.36 |
| 25 | KW-BMAP-18 [174]/DJ | KRKRWHW-GGLRSLGRKILRAWKKYG | 0.90/H | 1.00/1.00 | 0.98/0.98 | 1.0/1.0/1.0 | 0.88/0.98 | 0.58/0.68/1.3653 | 0.09 | −1.01 |
| 26 | Chrysophin-1-KW [175]/DJ | FFGWLIKGAIHAGKAIHGLI-GG- KRKRWHW |
0.59/L | 0.99/1.00 | 0.98/0.98 | 1.0/1.0/1.0 | 0.96/0.97 | 0.52/0.55 | 0.97 | −1.03 |
| 27 | KW-mastoparan [176]/DJ | KRKRWHW-GG-INLKALAALAKKIL | 0.90/L | 0.87/1.00 | 0.75/0.98 | 1.0/0.9/1.0 | 0.92/0.96 | 0.62/0.66 | 0.42 | −1.11 |
| 28 | KW-pleuricidin [177]/DJ |
KRKRWHW- GWGSFFKKAAHVGKHVGKAALTHYL |
0.66/L | 0.89/1.00 | 0.99/0.98 | 1.0/0.9/0.9 | 0.96/1.00 | 0.60/0.65 | 0.37 | −0.95 |
| 29 | MTD [178]/E | RRRRRRRRGRQ-KLLNLISKLF | 0.98/H | 0.28/0.60 | 0.58/0.96 | 1.0/0.6/0.9 | 0.73/0.97 | 0.67/0.70 | 0.06 | −1.06 |
| 30 | L-K6V1 temp [112]-KW-analog/DJ | IKKIVSKIRKLLKR-G-KRKRWHW | 0.95/H | 0.98/1.00 | 1.0/0.98 | 1.0/1.0/0.8 | 0.92/1.0 | 0.65/0.66/1.678 | 0.07 | −1.12 |
| 31 | T2R1 [88]-KW-analog1/DJ | RHHWRRYARIGFRAVRSVIGK- KTKRWHW |
0.92/H | 0.93/0.94 | 0.98/0.98 | 1.0/1.0/1.0 | 0.69/0.97 | 0.66/0.62/1.36406 | 0.03 | −1.09 |
| 32 | T2R1 [88]-KW-analog2/DJ | RHHWRRLARIGFRAVRSVIGK- KTKRWHW |
0.93/H | 0.96/0.87 | 0.97/0.98 | 1.0/1.0/1.0 | 0.60/0.97 | 0.67/0.62/1.5722 | 0.05 | −1.30 |
| 33 | KW-BMAP-18 [174]-analog1/DJ | KRKRWHW-GGLRSLGRKLLRAWKKYG | 0.91/H | 1.00/0.99 | 0.96/0.98 | 1.0/1.0/1.0 | 0.84/0.97 | 0.63/0.71/1.62236 | 0.08 | −1.05 |
| 34 | BP100 [155]-KW-analog/DJ | LKLFKKILKYLN-G-KRKRWHW | 0.93/H | 0.996/0.999 | 0.966/0.981 | 1.0/0.961/1.0 | 0.87/1.0 | 0.635/0.687/1.80571 | 0.894 | −1.32 |
| 35 | Zyk-1 [88]-WFR8-analog/DJ | GIGLEIVKKIILKIGKKIGRII-GG- RRWFRRRRRR |
0.83/L | 0.999/0.998 | 0.986/0.977 | 1.0/0.985/0.964 | 0.98/0.99 | 0.60/0.614/1.599 | 0.938 | −1.33 |
| 36 | KW-BMAP-18 [174]-analog2/DJ | KRKRWHW-GGLASLGRKLLRAWKKYG | 0.85/H | 0.988/0.986 | 0.951/0.982 | 1.0/0.971/1.0 | 0.85/0.97 | 0.684/0.708/1.85648 | 0.399 | −1.09 |
| 37 |
R8FW-GGGPPKG- temp [112] R9R14/DJ |
RRRRRRFWRR-GGGPPKG- IKKIVSKIRKLLKR |
0.95/H | 0.997/1.0 | 0.954/0.968 | 1.0/0.958/0.822 | 0.97/0.99 | 0.71/0.60/1.25133 | 0.032 | −1.18 |
| 38 |
R8FW-GGEPPKG- temp [112] R9R14/DJ |
RRRRRRFWRR-GGEPPKG- IKKIVSKIRKLLKR |
0.94/H | 0.998/0.988 | 0.926/0.973 | 1.0/0.96/0.996 | 0.96/0.944 | 0.70/0.596/1.51442 | 0.028 | −1.20 |
| 39 | R7A5FW-GGEPPKG temp [112]/DJ |
RRRRARFWRR-GGEPPKG- IKKIVSKIRKLLKR |
0.92/H | 0.998/0.968 | 0.916/0.973 | 1.0/0.9654/0.95 | 0.97/0.927 | 0.72/0.597/1.80807 | 0.010 | −1.25 |
| 40 | L-K6V1 temp. [112]-GGEPPKG-KW/DJ | IKKIVSKIKKLLK-GGEPPKG- KRKRWHW |
0.72/L | 0.971/0.986 | 0.996/0.955 | 0.994/0.985/0.97 | 0.94/0.839 | 0.50/0.624 | 0.007 | −1.15 |
| 41 | R8FW-GGGPPKG-IDR-1002 [9]/DJ |
RRRRRRFWRR-GGGPPKG- VQRWLIVWRIRK |
0.97/H | 1.0/0.944 | 0.181/0.979 | 1.0/0.862/0.854 | 0.71/0.40 | 0.633/0.606 | 0.064 | −1.17 |
| 42 | R8FW-GGGPPKG-IDR-1018 [9]/DJ |
RRRRRRFWRR-GGGPPKG- VRLIVAVRIWRR |
0.95/H | 1.0/0.978 | 0.344/0.980 | 1.0/0.863/0.982 | 0.79/0.33 | 0.612/0.602 | 0.039 | −1.17 |
| 43 | R8FW-GGGPPKG- IDR-1018-R6/DJ |
RRRRRRFWRR-GGGPPKG- VRLIVRVRIWRR |
0.96/H | 1.0/0.929 | 0.406/0.980 | 1.0/0.832/0.354 | 0.81/0.59 | 0.626/0.605 | 0.027 | −1.21 |
| 44 | R8FW-GGEPPKG-IDR-1018-R6/DJ |
RRRRRRFWRR-GGEPPKG- VRLIVRVRIWRR |
0.96/H | 1.0/0.702 | 0.356/0.983 | 1.0/0.854/0.99 | 0.78/0.21 | 0.612/0.607/1.16498 | 0.026 | −1.23 |
| 45 | R8FW-GGEPPKG-IDR-1018-L1R6/DJ |
RRRRRRFWRR-GGEPPKG- LRLIVRVRIWRR |
0.96/H | 1.0/0.939 | 0.277/0.983 | 1.0/0.86/0.04 | 0.77/0.338 | 0.623/0.622/1.43256 | 0.025 | −1.20 |
| 46 | Pexiganan-L18 [88]/E | GIGKFLKKAKKFGKAFVLILKK | 0.75/L | 0.997/1.0 | 1.0/0.976 | 0.46/0.299/0.22 | 1.0/1.0 | 0.598/0.661 | 0.892 | −0.94 |
| 47 | Flexampin [114]/E | GIKKWVKGVAKGVAKDLAKKIL | 0.56/L | 0.990/1.0 | 1.0/0.977 | 0.993/0.937/0.544 | 0.99/1.0 | 0.423/0.531 | 0.817 | −0.78 |
| 48 | Zyk-1 [88]/E | GIGREIIKKIIKKIGKKIGRII | 0.65/L | 0.978/0.998 | 0.998/0.97 | 1.0/0.933/0.946 | 0.88/1.0 | 0.526/0.662 | 0.583 | −0.86 |
| 49 | Adepantin-1A [88]/E | GIKKAVGKALKGLKGLLKALGES | 0.39 | 0.980/1.0 | 1.0/0.977 | 1.0/0.972/0.398 | 0.99/1.0 | 0.554/0.659/1.35587 | 0.17 | −1.51 |
| 50 | Novispirin [152]-KW-analog2/DJ | KNLRIFRKGIHIHKKY-GG- KRKRWHW |
0.903/H | 0.972/0.946 | 0.994/0.983 | 1.0/0.939/0.822 | 0.96/0.989 | 0.5884/0.6 | 0.195 | −1.63 |
| 51 |
WFR8-adepantin-1A-
analog2/DJ |
RRWFRRRRRR- GIKKAVGKALKGLKLLLKALGES |
0.878/L | 0.999/1.0 | 0.923/0.9485 | 1.0/0.987/0.908 | 0.96/0.983 | 0.616/0.622/1.62411 | 0.826 | −1.63 |
| 52 | KW-second-bovine- BMAP-18 [179]/DJ |
KRKRWHW-GRFKRFRKKFKKLFKKIS | 0.961/H | 0.999/1.0 | 0.995/0.981 | 1.0/0.845/1.0 | 0.91/1.0 | 0.565/0.698 | 0.176 | −1.09 |
* We used the servers listed in Table 2 and applied them in the same order. Highlighted peptides (bold name) with underlined activity scores are our selection for the designed peptides with the best overall score (see Table 6). Bold sequence segments have predicted or verified CPP activity. Underlined residues are optimal substitutions for increasing anti-inflammatory activity or decreasing peptide toxicity.