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. Author manuscript; available in PMC: 2022 Sep 22.
Published in final edited form as: Nat Struct Mol Biol. 2022 Jul 21;29(8):759–766. doi: 10.1038/s41594-022-00803-w

Extended Data Fig. 3 |. Image processing pipeline and validation of KaiC-EA.

Extended Data Fig. 3 |

a, Image processing pipeline for two combined KaiC-EA datasets: a 40° tilted dataset, and one collected on thin carbon. b, Local resolution estimation of cryo-EM reconstructions calculated by RELION52. c, Euler distribution plots depicting particle orientations present in final reconstructions. More populated views are colored in red. d, 3D Fourier Shell Correlation (3DFSC)53 of final nighttime state reconstructions, with a global resolution of 2.8 Å for the C6-state, and 3.2 Å for the C2-state at FSC = 0.143.