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. Author manuscript; available in PMC: 2022 Sep 22.
Published in final edited form as: Nat Struct Mol Biol. 2022 Jul 21;29(8):759–766. doi: 10.1038/s41594-022-00803-w

Extended Data Fig. 1 |. Image processing pipeline and validation of C6 symmetric KaiC-AE structure.

Extended Data Fig. 1 |

a, Image processing pipeline for KaiC-EA datasets with fluorinated fos-choline-8. b, Local resolution estimation of cryo-EM reconstruction calculated by RELION52. c, Euler distribution plots depicting particle orientations present in final reconstruction. More populated views are colored in red while less populated are depicted in blue. d, 3D Fourier Shell Correlation (3DFSC)53 of final daytime state reconstruction, with a global resolution of 3.8 Å at FSC = 0.143.