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Inductive response at pcalp dependent on the protocatechuate concentration in the medium. Cells of Acinetobacter sp. strain ADP197 with plasmid pAC17 were grown on mineral medium with pyruvate as the carbon source. Protocatechuate was added to the cultures in the middle of logarithmic growth, resulting in the concentrations indicated, and β-galactosidase activity was determined 60 min later.
