TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Acinetobacter strains | ||
| ADP1 | Wild type (strain BD413, ATCC 33305) | 29 |
| ADP331 | ΔpcaU2, a 552-bp deletion between NcoI and MluI restriction sites in pcaU | 20 |
| ADP197 | recA100::Tn5 | 21 |
| ADPU331 | ΔpcaU2 recA100::Tn5 | This study |
| E. coli strains | ||
| K-12 | Wild type | |
| DH5α | general cloning strain | 23 |
| S17-1 | Mobilizing strain | 50 |
| Plasmids | ||
| pRK415 | Tcr, lacp/o, shuttle vector | 30 |
| pUC19 | Apr, lacp/o | 55 |
| pKOK6 | Contains lacZ-Kmr cassette for construction of transcriptional fusions | 32 |
| pPHU234 | Tcr, broad-host-range translational lacZ fusion vector | 27 |
| pZR9 | pcaU′-pcaIJFBD′ from Acinetobacter sp. strain ADP1 | 20 |
| pAC3 | 798-bp NarI fragment from pZR9 inserted into the AccI site of pUC19, lacp downstream of pcaI′ | This study |
| pAC4 | 798-bp NarI fragment from pZR9 inserted into the AccI site of pUC19, lacp upstream of pcaI′ | This study |
| pAC5 | lacZ-Kmr cassette from pKOK6 inserted into PstI site of pRK415, lacp downstream of lacZ | This study |
| pAC12 | 740-bp XbaI fragment from pAC4 inserted into XbaI site of pRK415, lacp downstream of lacZ | This study |
| pAC15 | lacZ-Kmr cassette from pKOK6 inserted into PstI site of pAC12, transcriptional pcaU-lacZ fusion, lacp downstream of lacZ | This study |
| pAC16 | 740-bp XbaI-PstI fragment from pAC3 inserted between XbaI and PstI sites of pRK415 | This study |
| pAC17 | lacZ-Kmr cassette from pKOK6 inserted into PstI site of pAC16, transcriptional pcaI-lacZ fusion, lacp downstream of lacZ | This study |
| pAC18 | 361-bp EcoRI-XhoI fragment from pZR9 inserted between EcoRI and XhoI sites of pPHU234, translational pcaU-lacZ fusion | This study |