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. 2022 Sep 19;11(9):1843. doi: 10.3390/antiox11091843

Table 2.

Sequences for real-time PCR primers.

Genes Gene Bank ID Primer Sequence (5-3) Product Length (bp)
IL-1β NM_204524.1 F TGCCTGCAGAAGAAGCCTCG 204
R GACGGGCTCAAAAACCTCCT
TNF-α NM204267 F TGTGTATGTGCAGCAACCCGTAGT 229
R GGCATTGCAATTTGGACAGAAGT
IFN-γ NM_205149.1 F ATGTAGCTGACGGTGGACCT 196
R TTCACGCCATCAGGAAGGTT
IL-4 NM_001007079.1 F GTGCCCACGCTGTGCTTAC 82
R AGGAAACCTCTCCCTGGATGTC
IL-10 NM_001004414.2 F GGAGCTGAGGGTGAAGTTTGA 129
R GACACAGACTGGCAGCCAAA
Occludin NM_205128.1 F CCGTAACCCCGAGTTGGAT 214
R ATTGAGGCGGTCGTTGATG
Claudin-1 NM_001013611.2 F AGGTGTACGACTCGCTGCTT 242
R AGCCACTCTGTTGCCATACC
Claudin-2 NM_001277622.1 F CCTGCTCACCCTCATTGGAG 144
R GCTGAACTCACTCTTGGGCT
ZO-1 XM_015278975.2 F TTTACATGATGACCGCCTGT 213
R GAATCCTCCCTAACGGGTTC
β-actin NM_205518 F TGCTGTGTTCCCATCTATCG 150
R TTGGTGACAATACCGTGTTCA