Figure 11.

Effects of chemotherapeutic drugs in combination with the OCF on AGS wt and resistant cells. Cell count after 72 h treatment. AGS wt cells were treated with vehicle/OCF 30 µM/OCF 60 µM ± IC₅₀ dose of 5-fluorouracil (12 µM)/Paclitaxel (4 nM)/cisplatin (10 µM) (a). 5FUr cells were treated with vehicle/OCF 30 µM/OCF 60 µM ± IC₅₀ dose of 5-fluorouracil (250 µM) (b). TAXr cells were treated with vehicle/OCF 30 µM/OCF 60 µM ± IC₅₀ dose of Paclitaxel (100 nM) (c). CISr cells were treated with vehicle/OCF 30 µM/OCF 60 µM ± IC₅₀ dose of cisplatin (100 µM) (d). Representation of the percentage increase in efficacy between combined treatments (IC₅₀ dose of drug and 60 µM OCF) vs the mono-treatment with the single IC₅₀ dose of the drug in the different cell lines obtained from the previous experiments is shown in this figure (e). Data are presented as mean ± SD of three independent experiments. Meaning of the following significance symbols is specified on top-right of the figure: *** p < 0.001, **** p < 0.0001, $ p < 0.05, $$ p < 0.01, $$$ p < 0.001, $$$$ p < 0.0001, ° p < 0.05, °° p < 0.01, °°°° p < 0.0001, £ p < 0.05, ££££ p < 0.0001, § p < 0.05, # p < 0.05 (Two-way ANOVA Tukey’s multiple comparisons test).