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. 2022 Sep 2;9(9):433. doi: 10.3390/bioengineering9090433

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Lpp-OmpA-ATRAM on the EcN/ΔtnaA::T7 RNAP membrane surface. (a) The growth curves of EcN/ΔtnaA::T7 RNAP/pLysS-LOA and EcN/ΔtnaA::T7 RNAP (WT). (b) SDS-PAGE gel photo showing expression levels of Lpp-OmpA-ATRAM over time. The black arrow indicates the target protein. (c) Lysis curves of EcN/ΔtnaA::T7 RNAP/ pET29a-α3-E after different induction starting points. (d) Confocal images of Lpp-OmpA-ATRAM on the engineered bacterial membrane surfaces. The green fluorescence is derived from the expression of Lpp-OmpA-ATRAM, scar bar is 30.9 μm.