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. 2022 Sep 13;11(9):1800. doi: 10.3390/antiox11091800

Table 3.

Real-time PCR primer sequences.

Genes a Forward primer (5′-3′) Reverse primer (5′-3′) Length Accession No.
TLR2 GCAGCCGCTTCAAAACTCAT GAACAAAGCCCTCAAAGCGG 105 NP_997977
MyD88 GTTGCGCTAAACATGAGCGT GTCTTCTCTGTCCAGCTCCG 237 A8QMS7
FADD GGCAGAAGATAACACGGCCT ATTTGCGGCCTAGTTTTCGC 200 NP_001373289
NF-κB TCACAGGGTCCTCGATGTCT CTGGCTGTTTGGAGACAGGT 78 NP_001001839
TGF-β CGTCTTCCAGCAAGCTCAGA TCCGAAGACGCAATTCTGCT 116 NP_878293
IL-10 CACAACCCCAATCGACTCCA GAGCAAATCAAGCTCCCCCA 175 NP_001018621
IL-8 GGAAGACCTGCCTCAATCCC GGGGCGGAGGTAGAATTTGG 118 XP_001342606
TNF-α GCAATCCGCTCAATCTGCAC GCAGCGCCGAGGTAAATAGT 74 NP_998024
Caspase8 ACCAGGACCTGCTGTCATTG TATCTGGAGATGCGCTGCTG 160 XP_685430
Bcl-2 GCGCTTCAACGCAGTCATAG GCAGCTAGACCAAAGACCGT 291 XP_001341214
Bcl-xl CAAGGAGGATGGGAACGCTT TTCTGTGCAATGAGTCCCCC 146 NP_571882
AP-1 CGTGAGTGTCACCTCGACTC GTCCTCATAAACCGGCGACT 127 NP_956281
Nrf2 CTGCCGTAAACGCAAGATGG ATCCGTTGACTGCTGAAGGG 287 NM_182889.1
Keap1 GGAAGTCACCCTTCGAGACG AGAGGACGTGAAGAACGCAG 107 NM_182864.2
CAT GGAAGAGGATGACGAAGAG GTTACGGCGAGATGATGT 232 NP_570987
SOD ACAGAAGAGAAGTATCAGGAG CACCGTAACAGCAGACAT 228 NP_956270
Hsp70 TCCATCACAAGGGCACGTTT CAGGGCTTTCTCAACTGGGT 78 Q91233.1
β-actin ACCCCATTGAGCACGGTATT GCTCCTCAGGGGCAACTCTC 96 KJ126772.1

aTLR2, Toll like receptor 2; MyD88, myeloid differentiation factor 8; FADD, Fas-associating protein with a novel death domain; NF-κB, Nuclear factor Kappa B; TGF-β, Transforming growth factor-β; IL-10, Interleukin 10; IL-8, Interleukin 8; TNF-α, Tumor necrosis factor-α; Caspase8, Cysteine-requiring aspartate protease 8; Bcl-2, B-cell lymphoma-2; Bcl-xl, B-cell lymphoma-xl; AP-1, Activating protein-1; Nrf2, Nuclear factor erythroid 2-related factor 2; Keap1, Kelch-like ECH-associated protein1; CAT, Catalase; SOD, Superoxide dismutase; Hsp70, Heat shock protein 70.