Figure 2.

Effect of hesperidin on the cell cycle arrest of prostate cancer cells. (A) Cell cycle distribution was analyzed after dose-dependent treatment of PC3 and DU145 cells with hesperidin via flow cytometry. (B) The ability of hesperidin to regulate the expression of proliferating cell nuclear antigen (PCNA) was analyzed via immunofluorescence using Alexa 488 (green). Then, 4, 6-diamino-2-phenylindole (DAPI; blue) was used to stain the nuclei of PC3 and DU145 cells. Scale bars of the first horizonal panels are 40 μm, and scale bars of the second horizonal panels are 20 μm. All results of the arithmetic mean (n = 3) denote statistical significance with asterisks (* p < 0.05 and ** p < 0.01).