Figure 4.

Effect of hesperidin on intracellular Ca²⁺ levels in prostate cancer cells. (A) Changes in intracellular Ca²⁺ concentrations after hesperidin treatment were determined via fluo-4 staining in PC3 and DU145 cells. (B) Changes in intracellular Ca²⁺ concentrations via co-treatment with hesperidin and 2-aminoethyl diphenyl borate (2-APB; 2 μM) were determined via fluo-4 staining in PC3 and DU145 cells. (C) Relative proliferation of PC3 and DU145 cells was verified after co-treatment with hesperidin and 2-APB for 48 h. All results of the arithmetic mean (n = 3) denote statistical significance with asterisks (* p < 0.05, ** p < 0.01, and *** p < 0.001).