Figure 5.

Effect of IQ-1L on LPS-induced c-Jun (Ser63) phosphorylation. Human MonoMac-6 monocytic cells were pretreated with indicated concentrations of IQ-1L or 0.5% DMSO for 30 min, followed by treatment with LPS (250 ng/mL) or control buffer for another 30 min. Controls with cells alone (no DMSO) or cells treated with LPS alone were also included. The cells were lysed, and the lysates were analyzed by Western blotting on 10% SDS-PAGE gels. After blotting for phospho-c-Jun, the same blots were stripped and reblotted for total JNK as a loading control for the lysates. Representative blots from three independent experiments are shown (A). The blots were analyzed by densitometry, as described under Materials and Methods, and the ratio of phospho-c-Jun/total c-Jun is shown in (B). Values are expressed as mean ± S.D. of three independent experiments. * p < 0.05 compared with DMSO + LPS-treated control cells.