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. 2022 Aug 25;10(9):2077. doi: 10.3390/biomedicines10092077

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Mice with adipocyte-specific LAMA4 deletion exhibit increased eWAT adipocyte area when maintained on a chow diet. Male Lama4^fl/fl (fl/fl) and Lama4^AKO (AKO) mice were provided a chow diet from weaning until 24 weeks of age; body weight was measured weekly and body composition was measured via NMR every other week. (a) Knockdown of Lama4 was confirmed in eWAT and iWAT; heart is shown as a positive control (n = 3–4 per genotype). Black bars represent Lama4^fl/fl mice and red bars represent Lama4^AKO mice. (b) There were no differences in body weight between Lama4^fl/fl (black) and Lama4^AKO mice (red) (n = 16–18 per genotype). (c) Adiposity did not differ between Lama4^fl/fl (black) and Lama4^AKO mice (red) (n = 16–18 per genotype). (d) Representative images of H&E-stained eWAT and iWAT (n = 4–6 per group). (e) Adipocyte area is significantly increased in eWAT, but not in iWAT, of chow-fed Lama4^AKO mice (n = 4–7 per genotype per tissue). Black triangles represent Lama4^fl/fl mice and red triangles represent Lama4^AKO mice. * p < 0.05 and ** p < 0.01 between genotypes. Scale bars = 250 μm.

Mice with adipocyte-specific LAMA4 deletion exhibit increased eWAT adipocyte area when maintained on a chow diet. Male Lama4^fl/fl (fl/fl) and Lama4^AKO (AKO) mice were provided a chow diet from weaning until 24 weeks of age; body weight was measured weekly and body composition was measured via NMR every other week. (a) Knockdown of Lama4 was confirmed in eWAT and iWAT; heart is shown as a positive control (n = 3–4 per genotype). Black bars represent Lama4^fl/fl mice and red bars represent Lama4^AKO mice. (b) There were no differences in body weight between Lama4^fl/fl (black) and Lama4^AKO mice (red) (n = 16–18 per genotype). (c) Adiposity did not differ between Lama4^fl/fl (black) and Lama4^AKO mice (red) (n = 16–18 per genotype). (d) Representative images of H&E-stained eWAT and iWAT (n = 4–6 per group). (e) Adipocyte area is significantly increased in eWAT, but not in iWAT, of chow-fed Lama4^AKO mice (n = 4–7 per genotype per tissue). Black triangles represent Lama4^fl/fl mice and red triangles represent Lama4^AKO mice. * p < 0.05 and ** p < 0.01 between genotypes. Scale bars = 250 μm.