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. 2022 Aug 24;11(9):1642. doi: 10.3390/antiox11091642

Figure 1.

Figure 1

Mitochondrial and neuronal abnormalities in sel-12 mutants are mediated through mitochondrial calcium. (A) Quantification of cytoplasmic calcium levels in animals expressing GCaMP, a genetically encoded calcium biosensor, and mKate, an expression control, in the TRNs. (n ≥ 25). (B) Quantification of mitochondria calcium levels in animals expressing mitochondrial-targeted GCaMP6 and mCherry, an expression control, in the TRNs. (n ≥ 21). (C) Representative image of healthy and aberrant ALM neurons (scale bar = 10 µm) and (D) quantification of the frequency of aberrant or health neurons. (n ≥ 20). (E) Representative image of continuous and discontinuous mitochondrial organization in the ALM soma (scale bar = 10 µm) and (F) quantification of the frequency of continuous and discontinuous mitochondria. (n ≥ 20). (G) Ratio of oxidized to non-oxidized roGFP1 in the mitochondria of ALM neurons as a quantitative measure of oxidation levels. (n ≥ 20). ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 using one-way ANOVA with Kruskal-Wallis multiple comparison test (A,B,G) or chi-square test (D,F). Comparisons are made to wild type unless otherwise indicated. Error bars indicate mean +/− SEM.