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. 2022 Aug 24;11(9):1642. doi: 10.3390/antiox11091642

Figure 3.

Figure 3

Inhibition of mTORC1 reduces mitochondrial ROS and rescues the hypersensitivity of sel-12 mutants to oxidative stress. (A) Relative mitochondrial oxidation levels in animals expressing mitochondria targeted roGFP1 in the TRNs. (n = 30). (B) Survival rate of animals following 24-h exposure to the oxidant paraquat (0, 50, 100, and 150 mM paraquat). (50 animals per strain, performed 3 times) (C) Paraquat survival curve in animals exposed to skn-1 or control RNAi. (D,E) Representative images (D) and quantification (E) of GFP fluorescence intensity in gst-4p::GFP transgenic animals as a reporter for transcription of SKN-1/Nrf2 target GST-4. ns p > 0.05, * p < 0.05, *** p < 0.001 using one-way ANOVA with Kruskal-Wallis test (A,D,E) or two-way ANOVA with Bonferroni test (B,C). Comparisons are made to wild type unless otherwise indicated. Error bars indicate mean +/− SEM.