Table 5.
HLA-Ia Ab profiles in the sera of normal non-alloimmunized male volunteers after purification of IgG using Protein-G affinity purification columns, elaborate the profile of the naturally occurring anti-HLA-I antibodies, suggesting that under natural conditions these antibodies may be masked by other factors, such as anti-idiotypic antibodies and immune complexes.
| Mr | Ho | Rn | Th | Sk | Vd | Me | Cr | Ad | ||
|---|---|---|---|---|---|---|---|---|---|---|
| NC (Albumin) | 862 | 2101 | 940 | 1652 | 5052 | 1451 | 874 | 2752 | 1326 | |
| Anti-HLA-A IgG profile after affinity purification | A*0101 | 631 | 1697 | 747 | 1255 | 1352 | 505 | 646 | 1895 | 804 |
| A*0201 | 661 | 1563 | 797 | 1209 | 1668 | 648 | 2172 | 878 | ||
| A*0203 | 712 | 1641 | 756 | 1155 | 1727 | 604 | 2064 | 886 | ||
| A*0206 | 789 | 1804 | 911 | 1324 | 2127 | 518 | 730 | 2650 | 965 | |
| A*0301 | 833 | 2197 | 911 | 1587 | 1898 | 555 | 733 | 2408 | 1018 | |
| A*1101 | 970 | 2645 | 1066 | 1639 | 2053 | 730 | 808 | 2892 | 1132 | |
| A*1102 | 741 | 1684 | 1056 | 1277 | 1662 | 579 | 637 | 2044 | 949 | |
| A*2301 | 897 | 2980 | 1133 | 1857 | 2007 | 688 | 1074 | 2841 | 1193 | |
| A*2402 | 1196 | 2967 | 1299 | 2284 | 2576 | 776 | 1175 | 3558 | 1437 | |
| A*2403 | 1016 | 2446 | 1108 | 1931 | 2261 | 687 | 894 | 2519 | 1238 | |
| A*2501 | 702 | 1847 | 804 | 1207 | 1576 | 505 | 693 | 2322 | 942 | |
| A*2601 | 1104 | 3401 | 1136 | 1976 | 2125 | 797 | 988 | 2721 | 1272 | |
| A*2901 | 902 | 2911 | 1013 | 1720 | 1640 | 778 | 892 | 2870 | 1090 | |
| A*2902 | 927 | 4136 | 1044 | 1781 | 2150 | 784 | 869 | 2832 | 1145 | |
| A*3001 | 1063 | 2421 | 1080 | 1751 | 3524 | 702 | 891 | 2540 | 1227 | |
| A*3002 | 686 | 1780 | 797 | 1286 | 1631 | 519 | 636 | 2077 | 896 | |
| A*3101 | 656 | 2074 | 741 | 1164 | 1746 | 615 | 2351 | 801 | ||
| A*3201 | 779 | 3294 | 866 | 1523 | 1533 | 660 | 772 | 2249 | 966 | |
| A*3301 | 994 | 2807 | 958 | 1761 | 2475 | 669 | 835 | 2668 | 1111 | |
| A*3303 | 752 | 2229 | 806 | 1357 | 1804 | 646 | 2177 | 891 | ||
| A*3401 | 1141 | 3851 | 1162 | 2004 | 2741 | 858 | 1121 | 2746 | 1272 | |
| A*3402 | 688 | 1675 | 778 | 1258 | 1546 | 513 | 663 | 2195 | 861 | |
| A*3601 | 881 | 1977 | 924 | 1510 | 1848 | 652 | 750 | 2257 | 1010 | |
| A*4301 | 1114 | 3782 | 1180 | 2081 | 2452 | 709 | 963 | 2308 | 1342 | |
| A*6601 | 924 | 3247 | 935 | 1575 | 2620 | 615 | 785 | 2061 | 1066 | |
| A*6602 | 983 | 3189 | 972 | 1759 | 3099 | 643 | 826 | 2226 | 1069 | |
| A*6801 | 651 | 1427 | 705 | 1236 | 1587 | 673 | 1914 | 775 | ||
| A*6802 | 932 | 2216 | 1065 | 1555 | 2094 | 989 | 750 | 3338 | 983 | |
| A*6901 | 1262 | 2777 | 1314 | 2237 | 2614 | 835 | 1138 | 2835 | 1521 | |
| A*7401 | 875 | 2719 | 897 | 1519 | 1734 | 602 | 755 | 1802 | 1025 | |
| A*8001 | 931 | 2885 | 1033 | 1676 | 2282 | 730 | 876 | 2141 | 1127 | |
| Anti-HLA-B IgG profile after affinity purification | B*0702 | 699 | 1549 | 825 | 1193 | 1792 | 657 | 2036 | 839 | |
| B*0801 | 796 | 2020 | 890 | 1447 | 1650 | 556 | 703 | 2576 | 975 | |
| B*1301 | 1331 | 3178 | 1356 | 2231 | 2725 | 817 | 1088 | 3103 | 1461 | |
| B*1302 | 967 | 2500 | 1096 | 1639 | 2385 | 660 | 837 | 2697 | 1126 | |
| B*1401 | 1198 | 2702 | 1231 | 2022 | 2203 | 950 | 1162 | 2876 | 1368 | |
| B*1402 | 977 | 2439 | 1093 | 1797 | 2176 | 748 | 919 | 2324 | 1093 | |
| B*1501 | 747 | 1602 | 860 | 1329 | 2026 | 718 | 2134 | 945 | ||
| B*1502 | 942 | 2610 | 1070 | 1764 | 2453 | 637 | 928 | 2745 | 1233 | |
| B*1503 | 986 | 2277 | 1039 | 1724 | 2208 | 595 | 739 | 2588 | 1130 | |
| B*1510 | 965 | 2934 | 1047 | 1714 | 2138 | 742 | 887 | 2357 | 1175 | |
| B*1511 | 1443 | 3526 | 1473 | 2466 | 2989 | 1010 | 1169 | 3462 | 1567 | |
| B*1512 | 751 | 2163 | 829 | 1496 | 2721 | 2523 | 3030 | 1147 | ||
| B*1513 | 1232 | 2917 | 1321 | 2105 | 2597 | 839 | 1097 | 2868 | 1455 | |
| B*1516 | 1205 | 4241 | 1389 | 2398 | 2725 | 1360 | 1199 | 3158 | 1503 | |
| B*1801 | 752 | 2024 | 853 | 1400 | 1641 | 565 | 658 | 2196 | 912 | |
| B*2705 | 595 | 1378 | 681 | 1084 | 2021 | 589 | 1990 | 764 | ||
| B*2708 | 551 | 1514 | 651 | 932 | 1669 | 520 | 2107 | 711 | ||
| B*3501 | 940 | 2199 | 1003 | 1667 | 2216 | 608 | 864 | 3054 | 1195 | |
| B*3701 | 1518 | 4054 | 1967 | 2613 | 2897 | 949 | 1259 | 3369 | 1540 | |
| B*3801 | 1191 | 2110 | 1095 | 1706 | 2060 | 621 | 853 | 2778 | 1160 | |
| B*3901 | 575 | 1291 | 657 | 1051 | 1446 | 520 | 1800 | 757 | ||
| B*4001 | 781 | 1980 | 910 | 1448 | 1761 | 520 | 698 | 3228 | 980 | |
| B*4002 | 624 | 1669 | 713 | 1124 | 1313 | 575 | 2044 | 783 | ||
| B*4006 | 1354 | 3682 | 1403 | 2280 | 2773 | 1006 | 1109 | 3287 | 1511 | |
| B*4101 | 760 | 1823 | 848 | 1413 | 1659 | 528 | 688 | 2299 | 940 | |
| B*4201 | 694 | 1603 | 765 | 1160 | 2014 | 632 | 2121 | 857 | ||
| B*4402 | 1121 | 2455 | 1196 | 1885 | 2067 | 710 | 2587 | 2575 | 1230 | |
| B*4403 | 811 | 2028 | 1044 | 1382 | 1972 | 515 | 2353 | 2474 | 932 | |
| B*4501 | 756 | 1814 | 786 | 1325 | 1780 | 500 | 2709 | 2221 | 918 | |
| B*4601 | 1090 | 2941 | 1466 | 2220 | 2396 | 898 | 1163 | 3297 | 1470 | |
| B*4701 | 879 | 2687 | 994 | 1729 | 1867 | 670 | 823 | 2567 | 1064 | |
| B*4801 | 1015 | 2291 | 1088 | 1769 | 1908 | 770 | 858 | 3066 | 1173 | |
| B*4901 | 929 | 2200 | 978 | 1725 | 2046 | 580 | 820 | 2569 | 1168 | |
| B*5001 | 675 | 1898 | 784 | 1207 | 1563 | 500 | 607 | 2152 | 880 | |
| B*5101 | 1118 | 2580 | 1214 | 2079 | 2259 | 724 | 973 | 2494 | 1355 | |
| B*5102 | 1116 | 2754 | 1140 | 1997 | 2271 | 842 | 976 | 2609 | 1341 | |
| B*5201 | 1050 | 3355 | 1227 | 1934 | 2261 | 744 | 982 | 2391 | 1299 | |
| B*5301 | 1251 | 3024 | 1359 | 2264 | 2474 | 845 | 1100 | 2725 | 1469 | |
| B*5401 | 1011 | 2528 | 1101 | 1737 | 2264 | 748 | 859 | 2661 | 1192 | |
| B*5501 | 955 | 2327 | 1020 | 1629 | 2089 | 599 | 806 | 2427 | 1111 | |
| B*5601 | 907 | 2698 | 1025 | 1594 | 2244 | 553 | 843 | 2667 | 1186 | |
| B*5701 | 762 | 2155 | 937 | 1512 | 2081 | 538 | 723 | 2144 | 990 | |
| B*5703 | 995 | 2740 | 1171 | 1864 | 2345 | 746 | 953 | 2789 | 1218 | |
| B*5801 | 1338 | 3218 | 1424 | 2331 | 3213 | 924 | 1185 | 2930 | 1523 | |
| B*5901 | 1155 | 2730 | 1207 | 1960 | 2502 | 766 | 1002 | 2600 | 1377 | |
| B*6701 | 661 | 1601 | 827 | 1180 | 1895 | 709 | 700 | 2412 | 841 | |
| B*7301 | 842 | 2326 | 960 | 1590 | 2458 | 555 | 932 | 2132 | 1085 | |
| B*7801 | 1150 | 2527 | 1186 | 1964 | 2544 | 743 | 948 | 2538 | 1346 | |
| B*8101 | 910 | 2241 | 1117 | 1658 | 2130 | 649 | 790 | 3022 | 1100 | |
| B*8201 | 2293 | 2886 | 1288 | 2311 | 2168 | 864 | 1006 | 2819 | 1445 | |
| Anti-HLA-C IgG profile after affinity purification | C*0102 | 1740 | 4894 | 1735 | 2972 | 2873 | 1221 | 1397 | 3892 | 1684 |
| C*0202 | 1941 | 4568 | 1894 | 3222 | 3936 | 1410 | 1504 | 3505 | 2011 | |
| C*0302 | 1830 | 4391 | 1829 | 3077 | 3369 | 1169 | 1512 | 3621 | 1967 | |
| C*0303 | 1663 | 3828 | 1602 | 2839 | 2851 | 1182 | 1409 | 3076 | 1849 | |
| C*0304 | 1900 | 4334 | 1801 | 2974 | 3272 | 1234 | 1538 | 3437 | 1977 | |
| C*0401 | 2166 | 6468 | 2126 | 3870 | 3863 | 1587 | 1897 | 3951 | 2305 | |
| C*0501 | 1967 | 3806 | 1726 | 3015 | 3404 | 1216 | 1912 | 3560 | 1886 | |
| C*0602 | 2165 | 4446 | 2175 | 3835 | 3672 | 1627 | 1732 | 4236 | 2108 | |
| C*0702 | 2551 | 5861 | 2388 | 4231 | 4327 | 1721 | 2066 | 4513 | 2605 | |
| C*0801 | 1510 | 3273 | 1467 | 2541 | 2896 | 1004 | 1218 | 3105 | 1555 | |
| C*1203 | 1588 | 3638 | 1551 | 2763 | 3129 | 1279 | 1382 | 3905 | 1674 | |
| C*1402 | 2077 | 5509 | 2009 | 3508 | 3440 | 1425 | 1640 | 3862 | 2073 | |
| C*1502 | 1408 | 3095 | 1375 | 2598 | 2614 | 1086 | 1183 | 3487 | 1481 | |
| C*1601 | 1724 | 4233 | 1739 | 3118 | 3473 | 1363 | 1526 | 3727 | 1967 | |
| C*1701 | 1857 | 4062 | 2103 | 3552 | 3823 | 1368 | 1625 | 3703 | 1991 | |
| C*1802 | 2275 | 4848 | 2292 | 3959 | 3990 | 1632 | 2054 | 4296 | 2353 |
The change in the number and strength of sera IgG antibodies against albumin, HLA-A, HLA-B and HLA-C, after affinity purification. Compare with Table 4 which shows the number and strength of these antibodies in raw sera. * Suggesting that the individuals may possibly carry naturally occurring antibodies formed against most commonly shared epitopes.