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. 2022 Sep 6;11(9):1761. doi: 10.3390/antiox11091761

Figure 8.

Figure 8

Effects of withagenin A diglucoside (WAD) (3) on apoptosis signaling pathways in human hepatocellular carcinoma (HepG2) cells. HepG2 cells were treated with WAD (50 and 100 μM) for 24 h. Cells were treated with culture media containing 0.5% DMSO as vehicle controls: (A) Protein expression levels of cleaved caspase-8, Bcl-2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), cleaved caspase-9, cleaved caspase-3, cleaved poly(ADP-ribose) polymerase (cleaved PARP), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (B–G) Each bar graph presents the densitometric quantification of protein expression levels. Data are presented as the mean ± standard error of the mean (SEM). n = 3, * p < 0.05 compared with the control.