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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
A possible implementation for the incorporation of CRISPR memory. Insertion of sub-sequences that are unique to the memory system of unwanted hosts in order to promote selective cleavage and digestion of the plasmid only in unwanted hosts.