Figure 1. iNKT cell development is severely impaired in T-Mettl14^−/− mice.

(A) m⁶A level in total mRNA of CD4⁺, CD8⁺ T cells, and iNKT cells (naive and activated) (n = 5–6).

(B) Immunoblot of METTL14 and METTL3 in total thymocytes and TCRβ⁺ splenocytes in WT and T-Mettl14^−/− mice. Data representative of three independent experiments.

(C) Representative staining of lymphocytes in indicated organs from WT and T-Mettl14^−/− mice with CD1d/PBS57 tetramer or unloaded CD1d tetramer.

(D and E) Summary of frequencies and cell numbers of iNKT cells in the indicated organs from WT and T-Mettl14^−/− mice (n = 5–8). SEM is shown. *p < 0.05, **p < 0.01, ***p < 0.001.