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. Author manuscript; available in PMC: 2022 Sep 22.
Published in final edited form as: Cell Rep. 2022 Aug 2;40(5):111156. doi: 10.1016/j.celrep.2022.111156

Figure 2. Defective development of iNKT cells in T-Mettl14−/− mice is cell intrinsic.

Figure 2.

(A) Representative histogram of CD1d expression on DP thymocytes. CD1d was stained with α-CD1d or isotype control antibody (n = 8).

(B) IL-2 detected by ELISA following 48-h co-culture of iNKT cell hybridoma DN32.D3 with irradiated thymocytes pulsed with α-GalCer ranging from 200 ng/mL to 12.5 ng/mL. Data representative of three independent experiments.

(C) Flow cytometric analysis of iNKT cells in the Jα18−/− recipient mice after 6 weeks of reconstitution with 1:1 mixture of bone marrow cells from WT (CD45.1) and T-Mettl14−/− (CD45.2).

(D) Quantification of iNKT cell reconstitution in thymus, spleen, and liver in bone marrow chimera recipients (n = 7). SEM is shown. ***p < 0.001.