TABLE 1.
Bacteria and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli XL1-Blue | F−recA1 end gyrA96 thi-1 hsdR17 supE44 relA1 lac[F′ proAB lacIZΔM15 Tn10(Tetr)] | Stratagene |
| Prochlorococcus sp. strain PCC 9511 | Axenic strain | 31 |
| Plasmids | ||
| pGEM-T | Apr cloning vector, dT-tailed EcoRV site | Promega |
| pPCCdnaA540 | pGEM-T with 540-bp dnaA PCR fragment cloned into the dT-tailed EcoRV site | This study |
| pPCCrnpB | pGEM-T with 300-bp rnpB fragment cloned into the dT-tailed EcoRV site | A. Schön |
| pMOSBlue | Apr cloning vector, dT-tailed EcoRV site | Amersham |
| pPCCftsZ571 | pMOSBlue with 571-bp ftsZ PCR fragment cloned into the EcoRV site | This study |
| pBluescript SK(+) | Apr cloning vector | Stratagene |
| p4K13 | 4,790-bp HindIII fragment containing ddlB (partial), ftsQ, ftsZ, panB, and a part of hemN cloned in pBluescript | This study |
| pPCCZAP4372 | pBluescript SK(−) 4,372-bp fragment containing ORF1 (partial), ORF2, ORF3, dnaA, and a part of ORF4 | This study |