Figure 1.

Decreased mitochondrial mass induced by deoxycholic acid (DCA) and cholic acid (CA) in muscle fibers involves a TGR5-dependent mechanism. Extensor digitorum longus (EDL) muscle fibers from WT and TGR5^−/− mice were incubated with DCA or CA for 72 h. Then, indirect immunofluorescence for TOM20 was performed. (a) Representative images for TOM20. Scale bar = 50 μm. (b,c) Analysis of TOM20 immunostaining to quantify mitochondrial mass expressed as a percentage in WT (b) or TGR5^−/− (c) fibers. Values correspond to the means ± SEM (n = 3, * p < 0.05 vs. control condition; one-way ANOVA and Tukey’s multiple comparison test).