Figure 5.

Deoxycholic acid (DCA) and cholic acid (CA) decrease oxidative phosphorylation (OXPHOS) complexes I and II from mitochondria in muscle fiber through a TGR5-dependent mechanism. Skeletal muscle fibers from WT and TGR5^−/− mice were incubated with DCA or CA for 72 h. Protein levels of mitochondrial complexes I, II, III, and V were detected by Western blot analysis. (a–c) Analysis of WT fibers incubated with DCA (a,b) or CA (a,c). (d–f) Analysis of TGR5^−/− fibers incubated with DCA (d,e) or CA (d,f). Values correspond to the mean ± SEM (n = 3, * p < 0.05, t-test).