Figure 6.

Increased mitochondrial reactive oxygen species (ROS) levels induced by deoxycholic acid (DCA) and cholic acid (CA) in muscle fibers involve a TGR5-dependent mechanism. Skeletal muscle fibers from WT and TGR5^−/− mice were incubated with DCA or CA for 72 h. Then, fibers were incubated with a Mitosox probe. (a) Representative images for the Mitosox signal (upper panel, lower panel zoom). Scale bar = 20 μm. (b,c) Analysis of the Mitosox signal normalized by the TMRE signal in WT (b) or TGR5^−/− (c) fibers. Values are expressed as the fold of induction and correspond to the means ± SEM (n = 3, * p < 0.05 vs. control condition; one-way ANOVA and Tukey’s multiple comparison test).