Figure 1.

Upregulation of H₂S-producing and H₂S-metabolizing enzymes occurs during the accumulation of ‘driver’ pathway mutations in human intestinal epithelial cell organoids. Western blot analysis (A) and densitometric quantification (B) of H₂S-producing (CBS, 3-MST, CSE) and H₂S-metabolizing (TST, ETHE1, SQR) enzymes in NL, A, AT, AKST, Ade^CIN TS and Ade^CIN TSK organoids after 7 days in culture. Data represent mean ± SEM of at least 5 independent experiments. * p < 0.05, ** p < 0.01 compared to control.