Figure 6.

Effect of pharmacological inhibition of 3-MST on the expression of various H₂S-producing and H₂S-metabolizing enzymes in Ade^CIN TS and Ade^CIN TSK organoids. (A,B) Western blot analysis of H₂S-producing and H₂S-metabolizing enzymes in Ade^CIN TS organoids in presence of increasing concentrations of HMPSNE. (C,D) Western blot analysis of H₂S-producing and H₂S-metabolizing enzymes in Ade^CIN TSK organoids in presence of increasing concentrations of HMPSNE. Analysis was performed at 48 h. CBS refers to the 45 kDa, truncated form, which was the CBS isoform predominantly present in these organoids. Data represent mean ± SEM of at least 4 independent experiments. * p < 0.05, ** p < 0.01 compared to control.