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. 2022 Aug 31;11(9):1736. doi: 10.3390/antiox11091736

Figure 4.

Figure 4

NO reverses the osteogenic phenotype induced by ILK silencing in hVECs. (A) Top: Scheme of the experimental design. Below: Western blot analysis (left) and quantification (right) of RUNX2 and BMP2. n = 8. * p < 0.05; *** p < 0.0001. (B) Alizarin Red staining. hVECs cultured with control medium or pro-osteogenic medium for 15 days were transfected with siSc or siILK for the last 7 days and treated with DETA-NO for 7 days. DETA-NO treatment was replenished every other day. Scale bar = 100 μm. Below: Quantification of alizarin red positive area. n = 6. Pro-calcific medium: *** p < 0.0001 vs. Si Sc; ## p < 0.001 vs. Si ILK.