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. 2022 Aug 31;11(9):1736. doi: 10.3390/antiox11091736

Figure 5.

Figure 5

Silencing of ILK in hVECs induced EnMT through the TGF/SMAD2/3 axis in a nitric-oxide-dependent manner. (A,B) hVECs transfected with siSc or siILK for 5 days. (A) Western blot analysis and quantification of phospho-Smad2 and phospho-Smad3. *** p < 0.0001 vs. Si Sc; ** p < 0.001 vs. Si Sc (B) Immunofluorescence of phospho-Smad2 (green) and TGF-β (red) and quantification of fluorescence intensity (right). n = 6. ** p < 0.001 vs. Si Sc. (C,D) hVECs transfected with siSc or siILK and treated with DETA-NONOate for 5 days. Quantification of nuclear translocation (right). n = 6. ** p < 0.001 vs. Si Sc. (C) Confocal fluorescent microscopy images of phospho-Smad2. (D) Western blot analysis of phospho-Smad2 and quantification. n = 6. ** p < 0.001 vs. Si Sc; @ p < 0.001 vs. Si ILK.