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. 2022 Aug 31;11(9):1736. doi: 10.3390/antiox11091736

Figure 6.

Figure 6

eNOS knock-out mice exhibited valve calcification, which correlates with low levels of ILK expression and increased RunX2 in valve endothelium. (A) Confocal microscopy image of endothelial marker IB4 in green and eNOS in red (left panel); ILK in green and endothelial marker CD31 in red (central panel); and ILK in green and Runx2 in red (right panel) in eNOS KO mice vs. WT. On the left, quantitation of ILK/RUNX2 colocalization in valve endothelium. N = 8. *** p < 0.0001 vs. WT. (B) Von Kossa staining in aortic valves of WT and eNOS KO mice. Scale bar = 50 μm. A quantification of VK positive area with respect to total area is shown below. n = 11. *** p < 0.0001 vs. WT.