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. 2022 Aug 26;11(9):1271. doi: 10.3390/biology11091271

Figure 3.

Figure 3

BMEC-conditioned media have a PCa-cell-type-specific effect on cell viability, not present in ECs of other tissues. Pre-clustered C4-2B cells were treated with BMEC-conditioned media (B-CM), C4-2B-conditioned media (C-CM), or conditioned media from BMEC cells that were treated with C4-2B-conditioned media (CtoB-CM). Cells were stained with calcein AM, ethidium homodimer, and Hoechst and imaged by confocal microscopy (a). IMARIS analysis of confocal z-stacks indicates a decrease in total cell number (b) and percent viability (c) on day 14 in conditions containing BMEC-conditioned media. Graphs represent means + SD. Statistical significance using two-way ANOVA with Bonferroni posttest, * p < 0.05, ** p < 0.01. Pre-clustered C4-2B cells were treated with HMEC-1-conditioned media (D-CM) or C4-2B-conditioned media (C-CM) (d) and HULEC-5a-conditioned media (L-CM) or C4-2B-conditioned media (C-CM) (e). Pre-clustered 22Rv1 (f) or PC3 (g) cells were treated with BMEC-conditioned media (B-CM) or the conditioned media from 22Rv1 (R-CM) or PC3 (P-CM), respectively.