Figure 4.

BMEC-conditioned media increases the number of Casp3+ cells. B-CM- and C-CM-treated cultures were fixed and stained for Ki67 and cleaved Casp3 (a). IMARIS analysis confirms an increase in Casp3+ cells on day 14 in B-CM (b), with a slight but not significant decrease in Ki67 (c). Accordingly, the Ki67/Casp3 ratio is different between the two conditions, with B-CM Ki67/Casp3 being near 1 (d). Scale bar = 50µm. Graphs represent means + SD. Statistical significance using two-tailed Student’s t-test, * p < 0.05, ** p < 0.01.