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TUNEL assay of conditioned medium (CM)-treated C4-2B clusters. Colorimetric TUNEL assay of C4-2B clusters cultured in microwells and treated with cancer cell CM (C-CM), HS27A-only CM (H-CM), BMEC and HS27A CM (BH-CM), and BMEC only CM (B-CM) on days 3, 7, and 14. Dark brown spots indicate cells that are undergoing apoptosis (a). Size in the pixel area of stain (dark brown) over total cluster area for each treatment group (b).
