Figure 6.

BMEC-conditioned media selectively suppresses phosphorylation of Rb and expression of Smac and FOXM1 in C4-2 cells. RPPA analysis of BMEC (B-CM)-, HMEC (D-CM)-, and C4-2 (C-CM)-conditioned medium treatment of C4-2B cells showed differential expression of signaling molecules pRb, Smac, and FOXM1 (downregulated proteins = green; upregulated proteins = red) (a). B-CM treatment showed differential expression of Smac (b), pRb (c), and FOXM1 (d) across time and between control treatment groups (D-CM and C-CM) quantified via densitometry below each corresponding blot.