. 2022 Sep 4;11(9):1311. doi: 10.3390/biology11091311

Table 1.

Specific primers used for the qPCR.

No	Specificity	Primer Name	Primer Type	Sequence (5′-3′)	GC Content (%)	Melting Temp (°C)	Ref.
1	Lactobacillus delbrueckii subsp. bulgaricus	LdelbF	Forward	GGRTGATTTGTTGGACGCTAG	47.6	66.9	[31]
1	Lactobacillus delbrueckii subsp. bulgaricus	LdelbR	Reverse	GCCGCCTTTCAAACTTGAATC	47.6	66. 7	[31]
2	Streptococcus thermophilus	S. thermophilusF	Forward	TTATTTGAAAGGGGCAATTGCT	36.3	65.2	[32]
2	Streptococcus thermophilus	S. thermophilusR	Reverse	GTGAACTTTCCACTCTCACAC	47.6	58.8	[32]
3	Bifidobacterium animalis	IDB61F	Forward	GCATGTTGCCAGCGGGTGA	63.1	73.1	[33]
3	Bifidobacterium animalis	IDBC1R	Reverse	ATCCGAACTGAGACCGGTT	52.6	63.5	[33]