Table 1.
Overview of studies examining oxidative DNA damage following SCI.
| Study | Animal Model | SCI Model | Analysis Method | Time Post-Injury | Effect Size (SCI-Sham) 1 |
Measurement Unit | Fold Change (SCI/Sham) 1 |
|
|---|---|---|---|---|---|---|---|---|
| Leski, 2001 [50] |
Rat | T13 weight-drop 50–75 g·cm |
HPLC with ECD | 1 hpi 3 hpi 6 hpi 12 hpi 1 dpi 2 dpi |
0.5 1.1 * 0.9 * 0.1 0.1 0.1 |
8-oxodG/2-dG × 10−4 | 1.4 1.8 * 1.6 * 1.1 1.1 1.1 |
|
| Sakurai, 2003 [46] |
Rabbit | Transient spinal cord ischemia |
8-oxodG IHC | 8 hpi 1 dpi 2 dpi |
No # Up # Up # |
8-oxodG immunoreactivity |
||
| Bao, 2004 [47] |
Rat | T4 extradural compression via aneurysm clip for 60 s |
8-oxodG IHC | 1 dpi | 24 * | 8-oxodG+ cells/0.4 mm2 |
30 * | |
| Takahashi, 2004 [37] |
Rabbit | Transient spinal cord ischemia |
8-oxodG IHC | 8 hpi 1 dpi 2 dpi |
No # Up # Up # |
8-oxodG immunoreactivity |
||
| Martin, 2005 [36] |
Rat | Sciatic nerve avulsion |
8-oxodG IHC | 2 dpi 4 dpi 5 dpi 7 dpi 10 dpi |
59 * 129 * 357 * 292 * 194 * |
8-oxodG+ motor neurons |
2.6 * 3.5 * 5.5 * 6.9 * 4.0 * |
|
| Xu, 2005 [29] |
Mouse | T12-L3 compression 20 g for 5 min |
8-oxodG IHC | 1 hpi 6 hpi 12 hpi 1 dpi |
Up # Up # Up # Up # |
8-oxodG immunoreactivity |
||
| King, 2006 [48] |
Rat | T7-9 hemi-section | 8-oxodG IHC | 7 dpi | 15 † (dorsal) 10 † (ventral) |
8-oxodG+ cells/mm2 | ||
| Huang, 2007 [10] |
Rat | T12 compression 50 g for 5 min |
8-oxodG IHC | 3 dpi 7 dpi |
10 † 4 † |
8-oxodG+ cells | ||
| Kotipatruni, 2011 [49] |
Rat | T10 weight-drop 10 g at 1.25 cm |
8-oxodG WB |
1 dpi 3 dpi 7 dpi 14 dpi 21 dpi |
0.36 * 0.44 * 0.57 * 0.41 * 0.32 * |
AU | 2.5 * 2.8 * 3.4 * 2.7 * 2.3 * |
|
| Sakarcan, 2017 [34] |
Rat | T7-10 weight-drop 10 g at 10 cm |
8-oxodG ELISA | 7 dpi | 4.5 * | ng/mg DNA | 2.1 * |
Color bars correlate with data shown in Figure 1B. 1 Estimates of effect size and fold change extracted from graphs in papers. * Statistical difference between SCI and Sham. # No statistics performed. † Not versus Sham (SCI count only). T13, thoracic spine level 13; L3, lumbar spine level 3; HPLC, high-pressure liquid chromatography; ECD, electrochemical detection; IHC, immunohistochemistry; WB, Western blot; hpi, hours post-injury; dpi, days post-injury; AU, arbitrary units.