Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 6;10:972017. doi: 10.3389/fcell.2022.972017

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Petrusová, Manning, Kubovčiak, Kolář and Filipp.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Identification of SCs from ST single cell suspension. (A) A single cell suspension generated from ST was incubated with FSHr-PE and Occludin-488 antibody. All FSHr⁺ cells positive for Occludin were sorted (right panel, red gate). Sorting gate contains 97,3% of cells. (B) To confirm specificity, double positive cells were analyzed by immunofluorescence microscopy to determine the presence of the transcription factor (TF), SOX9. (C) Immunofluorescence analysis of FSHr⁺Occludin⁺ double positive cell population cultured on a Petri dish confirming homogenity in the expression of SOX9 TF.