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Primer sequences for RT–qPCR.
| Primer Name | Sequence (5′–3′) | Size of PCR Amplicon (bp) |
|---|---|---|
| TLR-4-F | GTAGTTCTTTTGTCATTCTTGGTT | 122 |
| TLR-4-R | TGACCCAATCTTCATCATAGC | |
| TNF-α-F | CGCGACTGACACTGAAGACC | 79 |
| TNF-α-R | GCAGGAGTTCTGTGGTGGTG | |
| MYD88-F | TGACAGCCTACACCCTT | 166 |
| MYD88-R | GATGCCGTGGCGACTA | |
| IL-11-F | CCACAGAGATTGATCACCATAGG | 191 |
| IL-11-R | TGTCAGCTTTGGTACTGAGC | |
| IL-10-F | GTTATTAAAGCCATGGGAGAGC | 198 |
| IL-10-R | GAAGTCCATTTGTGCCATATCC | |
| β-actin-F | CTCCCCTCAATCCCAAAGCCAA | 127 |
| β-actin-R | ACACCATCACCAGAATCCATCA |
