Figure 2.

Anti-inflammatory effects of DLA. A 96-well plate was seeded with cells (2 × 10⁵ cells/mL) and incubated for 24 h. Following this, the cells were exposed to the DLA (12.5–50 µM) or vehicle alone for 20 h and/or for 3, then to LPS (500 ng/mL) for 24 h of incubation. Effect of DLA in LPS-induced RAW 264.7 macrophages on the production of NO (A) and prostaglandin E₂ (PGE₂) (B) and on the mRNA expression of inducible nitric oxide synthase (iNOS) (C) and cyclooxygenase-2 (COX-2) (D); protein expression of iNOS and COX-2 (E). To evaluate the quantification of the relative band intensities, densitometric analysis was performed; # p < 0.05 vs. treatment with the vehicle control; ** p < 0.05 vs. treatment with LPS alone. ns: non-significant vs. treatment with vehicle control.