. 2022 Aug 30;11(9):1699. doi: 10.3390/antiox11091699

Table 4.

Effects of maternal zinc on oxidative indicators and HSP70 in embryos under heat stress (mean ± S.E.).

Temperature	Maternal Zinc	ROS, nmol/mg port	MDA, nmol/mg port	PC, nmol/mg port	8-OHdG, nmol/mg port	HSP70, ng/g
37 °C ¹	Contro	1396 ± 56.61	0.60 ± 0.05 ^bc	0.99 ± 0.19 ^bc	14.14 ± 0.91 ^cd	14.14 ± 0.91 ^cd
	ZnSO₄	1325 ± 61.28	0.58 ± 0.02 ^bc	0.88 ± 0.13 ^cd	14.09 ± 0.52 ^cd	14.09 ± 0.52 ^cd
	Zn-Gly	1196 ± 52.02	0.53 ± 0.07 ^c	0.71 ± 0.05 ^d	13.08 ± 0.45 ^d	13.08 ± 0.45 ^d
39.5 °C ¹	Control	1553 ± 48.64	0.97 ± 0.13 ^a	1.49 ± 0.14 ^a	33.08 ± 1.12 ^a	33.08 ± 1.12 ^a
	ZnSO₄	1544 ± 32.14	0.69 ± 0.01 ^b	1.17 ± 0.00 ^b	22.66 ± 0.84 ^b	22.66 ± 0.84 ^b
	Zn-Gly	1495 ± 74.95	0.56 ± 0.04 ^c	1.0 ± 0.04 ^bc	15.16 ± 1.45 ^c	15.16 ± 1.45 ^c
37 °C ²		1295 ± 102.68 ^b	0.57 ± 0.05 ^b	0.86 ± 0.17 ^b	13.77 ± 0.78 ^b	13.77 ± 0.78 ^b
39.5 °C ²		1531 ± 54.74 ^a	0.74 ± 0.19 ^a	1.22 ± 0.23 ^a	23.64 ± 7.74 ^a	23.64 ± 7.74 ^a
	Control ³	1475 ± 97.61 ^a	0.78 ± 0.22 ^a	1.24 ± 0.31 ^a	23.61 ± 10.17 ^a	23.61 ± 10.17 ^a
	ZnSO₄ ³	1436 ± 127.85 ^a	0.64 ± 0.06 ^b	1.02 ± 0.18 ^b	18.39 ± 4.64 ^b	18.39 ± 4.64 ^b
	Zn-Gly ³	1324 ± 169.28 ^b	0.54 ± 0.05 ^c	0.85 ± 0.16 ^c	14.12 ± 1.49 ^c	14.12 ± 1.49 ^c
p-value
Temperature		<0.001	<0.001	<0.001	0.012	<0.001
Maternal zinc		0.003	<0.001	<0.001	0.001	<0.001
Temperature × maternal zinc		0.108	0.002	0.216	0.975	<0.001

ROS, reactive oxygen species. MDA, malondialdehyde. PC, protein carbonyl 8-OHdG, 8-hydroxydeoxyguanosine. HSP70, heat shock protein 70. Different letters (a–d) represent significant differences between the groups. ¹ Each value represents the mean ± SE of 10 replicates (n = 10). ² Each value represents the mean ± SE of 30 replicates (n = 30). ³ Each value represents the mean ± SE of 20 replicates (n = 20).