Table 5.

Effects of maternal zinc on oxidative indicators and HSP70 in embryos under heat stress (mean ± S.E.).

Temperature	Maternal Zinc	T-SOD, U/mg port	CuZn-SOD, U/mg port	GSH-Px, U/mg port	CAT, U/mg port	T-AOC, U/mg port	MT, ng/g
37 °C 1	Control	93.26 ± 3.05 d	63.67 ± 0.79 c	73.52 ± 3.30 b	8.02 ± 0.46 bc	0.86 ± 0.07 c	93.98 ± 0.37 c
	ZnSO4	114.7 ± 4.18 ab	67.29 ± 1.51 b	76.32 ± 2.71 b	8.71 ± 0.5 ab	1.14 ± 0.05 ab	96.84 ± 0.41 b
	Zn-Gly	119.6 ± 4.91 a	71.01 ± 0.80 a	80.48 ± 0.16 a	9.10 ± 0.57 a	1.19 ± 0.07 a	102.49 ± 0.87 a
39.5 °C 1	Control	86.13 ± 1.74 e	60.86 ± 0.17 d	64.57 ± 0.44 d	6.84 ± 0.45 d	0.71 ± 0.05 d	79.54 ± 2.19 e
	ZnSO4	102.7 ± 1.08 c	65.87 ± 1.84 b	69.38 ± 1.45 c	7.52 ± 0.37 cd	0.84 ± 0.04 c	90.75 ± 0.54 d
	Zn-Gly	112.7 ± 3.73 b	70.57 ± 0.46 a	73.40 ± 1.29 b	8.46 ± 0.33 ab	1.05 ± 0.11 b	93.60 ± 2.35 c
37 °C 2		109.2 ± 12.66 a	67.32 ± 3.32 a	76.77 ± 3.71 a	8.61 ± 0.65 a	1.06 ± 0.16 a	98.63 ± 3.91 a
39.5 °C 2		100.7 ± 11.16 b	65.77 ± 4.31 b	69.12 ± 3.96 b	7.60 ± 0.78 b	0.87 ± 0.16 b	87.96 ± 6.64 b
	Control 3	89.69 ± 4.49 c	62.27 ± 1.62 c	69.04 ± 5.33 c	7.43 ± 0.76 c	0.78 ± 0.10 c	86.76 ± 8.03 c
	ZnSO4 3	107.8 ± 6.90 b	66.58 ± 1.69 b	72.85 ± 4.27 b	8.11 ± 0.76 b	0.99 ± 0.17 b	93.80 ± 3.36 b
	Zn-Gly 3	116.2 ± 5.45 a	70.79 ± 0.63 a	76.94 ± 3.96 a	8.78 ± 0.54 a	1.12 ± 0.11 a	99.16 ± 4.81 a
p-value
Temperature		<0.001	0.011	<0.001	0.001	<0.001	<0.001
Maternal zinc		<0.001	<0.001	<0.001	0.001	<0.001	<0.001
Temperature × maternal zinc		0.315	0.212	0.614	0.507	0.138	<0.001

T-SOD, total superoxide dismutase. CuZn-SOD, copper, and zinc superoxide dismutase. GSH-Px, glutathione peroxidase. CAT, Catalase. T-AOC, total antioxidant capacity. MT, metallothionein. Different letters (a–e) represent significant differences between the groups. ¹ Each value represents the mean ± SE of 10 replicates (n = 10). ² Each value represents the mean ± SE of 30 replicates (n = 30). ³ Each value represents the mean ± SE of 20 replicates (n = 20).