Table 6.
Effects of maternal zinc on hepatocellular mitochondrial membrane potential, apoptosis Index and caspase3 activity in embryos under heat stress (mean ± S.E.).
| Temperature | Maternal Zinc | MMP, mV | AI, % | Caspase3, U/mg Prot |
|---|---|---|---|---|
| 37 °C 1 | Control | 12.06 ± 0.18 | 2.64 ± 0.21 d | 56.35 ± 1.80 |
| ZnSO4 | 13.13 ± 0.33 | 1.43 ± 0.15 e | 52.82 ± 1.28 | |
| Zn-Gly | 14.93 ± 0.84 | 0.24 ± 0.03 f | 49.25 ± 1.46 | |
| 39.5 °C 1 | Control | 10.93 ± 0.60 | 7.38 ± 0.34 a | 68.17 ± 1.69 |
| ZnSO4 | 12.51 ± 0.31 | 5.60 ± 0.30 b | 63.59 ± 3.68 | |
| Zn-Gly | 13.68 ± 1.42 | 3.61 ± 0.24 c | 59.25 ± 2.81 | |
| 37 °C 2 | 13.53 ± 1.38 a | 1.44 ± 1.01 b | 52.81 ± 3.18 b | |
| 39.5 °C 2 | 12.37 ± 1.43 b | 5.53 ± 1.59 a | 63.67 ± 4.58 a | |
| Control 3 | 11.49 ± 0.74 c | 5.01 ± 2.46 a | 62.26 ± 6.66 a | |
| ZnSO4 3 | 12.82 ± 0.45 b | 3.52 ± 2.15 b | 57.44 ± 6.20 b | |
| Zn-Gly 3 | 14.39 ± 1.21 a | 1.92 ± 1.74 c | 54.25 ± 5.83 c | |
| p-value | ||||
| Temperature | 0.013 | <0.001 | <0.001 | |
| Maternal zinc | <0.001 | <0.001 | <0.001 | |
| Temperature × maternal zinc | 0.745 | <0.001 | 0.781 | |
MMP, Mitochondrial Membrane Potential. AI, Apoptosis Index. Different letters (a–f) represent significant differences between the groups. 1 Each value represents the mean ± SE of 10 replicates (n = 10). 2 Each value represents the mean ± SE of 30 replicates (n = 30). 3 Each value represents the mean ± SE of 20 replicates (n = 20).