. 2022 Aug 30;11(9):1699. doi: 10.3390/antiox11091699

Table 7.

Effect of maternal zinc on CuZn-SOD, MT, Nrf2, UCP and Bax mRNA abundance expression in embryos liver under heat stress (mean ± S.E.).

Temperature	Maternal Zinc	CuZn-SOD	MT	Nrf2	UCP	Bax
37 °C ¹	Control	0.64 ± 0.03 ^d	0.87 ± 0.06 ^c	0.93 ± 0.05	0.85 ± 0.13 ^d	1.37 ± 0.05 ^c
	ZnSO₄	0.85 ± 0.05 ^b	0.98 ± 0.03 ^c	1.12 ± 0.10	0.99 ± 0.11 ^d	0.99 ± 0.11 ^d
	Zn-Gly	1.19 ± 0.03 ^a	1.72 ± 0.18 ^a	1.23 ± 0.05	1.03 ± 0.02 ^d	0.95 ± 0.09 ^d
39.5 °C ¹	Control	0.60 ± 0.01 ^d	0.82 ± 0.05 ^c	1.12 ± 0.11	1.52 ± 0.15 ^c	2.43 ± 0.27 ^a
	ZnSO₄	0.71 ± 0.00 ^c	0.88 ± 0.10 ^c	1.33 ± 0.02	2.08 ± 0.26 ^b	2.08 ± 0.26 ^b
	Zn-Gly	1.16 ± 0.02 ^a	1.54 ± 0.09 ^b	1.51 ± 0.15	2.43 ± 0.27 ^a	1.52 ± 0.15 ^c
37 °C ²		0.88 ± 0.25 ^a	1.19 ± 0.41 ^a	1.09 ± 0.14 ^b	0.96 ± 0.12 ^b	1.09 ± 0.21 ^b
39.5 °C ²		0.86 ± 0.26 ^b	1.08 ± 0.35 ^b	1.32 ± 0.18 ^a	2.01 ± 0.45 ^a	2.01 ± 0.45 ^a
	Control ³	0.63 ± 0.03 ^b	0.85 ± 0.05 ^b	1.03 ± 0.13 ^c	1.18 ± 0.39 ^c	1.90 ± 0.61 ^a
	ZnSO₄ ³	0.78 ± 0.08 ^b	0.93 ± 0.09 ^b	1.24 ± 0.12 ^b	1.46 ± 0.61 ^b	1.46 ± 0.61 ^b
	Zn-Gly ³	1.18 ± 0.03 ^a	1.63 ± 0.16 ^a	1.37 ± 0.18 ^a	1.73 ± 0.79 ^a	1.23 ± 0.33 ^c
p-value
Temperature		<0.001	0.038	<0.001	<0.001	<0.001
Maternal zinc		<0.001	<0.001	<0.001	<0.001	<0.001
Temperature × maternal zinc		0.004	0.536	0.661	0.010	0.035

CuZn-SOD, copper and zinc superoxide dismutase. MT, metallothionein. Nrf2, nuclear factor erythroid 2-related factor 2. UCP, uncoupling protein. Bax; Bcl2-Associated X Protein. Different letters (a–d) represent significant differences between the groups. ¹ Each value represents the mean ± SE of 10 replicates (n = 10). ² Each value represents the mean ± SE of 30 replicates (n = 30). ³ Each value represents the mean ± SE of 20 replicates (n = 20).